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Sharonda LeBlanc

Assistant Professor

(919) 515 - 7342

Bio

Dr. LeBlanc earned her PhD in Nanoscale Science from UNC Charlotte in 2012 where she explored the effects of proximal electric fields on single quantum dot fluorescence using time-resolved confocal microscopy. She moved on to a Postdoc at UNC Chapel Hill (2014 – 2020) where she used single molecule Förster/fluorescence resonance energy transfer (smFRET) to study protein – nucleic acid interactions involved in DNA repair. As a postdoc, she spent half of her time working at NC State with Professor Weninger. Dr. LeBlanc joined the NC State Physics faculty as an Assistant Professor in Fall 2020.

Research Description

Dr. LeBlanc is fascinated by the large numbers of protein machines called enzymes that coordinate to carry out complex biological processes. Her research focuses on understanding the molecular details of essential pathways. The LeBlanc lab seeks to uncover precisely how individual proteins interact with their binding partners, how those interactions are modulated by dynamic conformational changes, and how enzymes fail in complex diseases such as cancer. Dr. LeBlanc’s research approach will utilize confocal microscopy with time-correlated single photon counting to study these fast biological processes at the single molecule level. Of particular interest are the protein – nucleic acid interactions involved in the ribosome assembly pathway. She is also interested in exploring biological sensing applications with quantum dots.

Selected Publications

Coordinated protein and DNA conformational changes govern mismatch repair initiation by MutS
Sharonda J. LeBlanc, Jacob W. Gauer, Pengyu Hao, Brandon Case, Miho Sakato, Manju M. Hingorani, Keith R. Weninger, Dorothy A. Erie
Nucleic Acids Research, (2018), doi:10.1093/nar/gky865

Single molecule FRET: A powerful tool to study intrinsically disordered proteins
Sharonda J. LeBlanc, Prakash Kulkarni, Keith R. Weninger
Biomolecules, (2018), doi:10.3390/biom8040140

Using Atomic Force Microscopy to Characterize the Conformational Properties of Proteins and Protein-DNA Complexes That Carry Out DNA Repair
Sharonda LeBlanc, Hunter Wilkins, Zimeng Li, Parminder Kaur, Hong Wang, Dorothy A. Erie
Methods in Enzymology, (2017), doi:10.1016/bs.mie.2017.04.004

Single Molecule FRET to Measure Conformational Dynamics of DNA Mismatch Repair Proteins
Jacob W. Gauer, Sharonda LeBlanc, Pengyu Hao, Ruoyi Qui, Brandon Case, Miho Sakato, Manju M. Hingorani, Dorothy A. Erie, Keith R. Weninger
Methods in Enzymology, (2016), doi:10.1016/bs.mie.2016.08.012

Fluorescence Modulation in single CdSe quantum dots by moderate applied electric fields
Sharonda J. LeBlanc, Mason R. McClanahan, Tully Moyer, Marcus Jones, Patrick J. Moyer
Journal of Applied Physics, (2014), doi:10.1063/1.4856315

Enhancement of Multiphoton Emission from Single CdSe Quantum Dots Coupled to Gold Films
Sharonda J. LeBlanc, Mason R. McClanahan, Marcus Jones, Patrick J. Moyer
Nano Letters, (2013), doi:10.1021/nl400117h